Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyclin A | activates Cdk2 | Homo sapiens | |
cyclin E | activates Cdk2 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
functional co-expression of CDK2 in 293A, 293T or U2OS cells with myc- or GST-tagged cyclin A or E and with FLAG-tagged human eEF2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
additional information | cyclin A-CDK2 shuttles from the cytoplasm to the nucleus and could phosphorylate eEF2 in interphase cells | Homo sapiens | - |
- |
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + eukaryotic elongation factor 2 | Homo sapiens | phosphorylation on Ser595 by Cdk2 | ADP + phosphorylated eukaryotic elongation factor 2 | - |
? | |
ATP + [elongation factor 2] | Homo sapiens | the enzyme phosphorylates Ser595. Ser595 phosphorylation varies during the cell cycle and is required for efficient Thr56 phosphorylation (by elongation factor 2 kinase) in vivo | ADP + [elongation factor 2]phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-TVSEESNVLCLSKSPNKHNRLYMKARPFF | - |
Homo sapiens | ADP + biotin-TVSEESNVLCLSKpSPNKHNRLYMKARPFF | phosphorylated on Ser595 | ? | |
ATP + eukaryotic elongation factor 2 | phosphorylation on Ser595 by Cdk2 | Homo sapiens | ADP + phosphorylated eukaryotic elongation factor 2 | - |
? | |
ATP + eukaryotic elongation factor 2 | phosphorylation on Ser595 by Cdk2. Substrate mutant S595A is poorly phosphorylated by cyclin A-CDK2 | Homo sapiens | ADP + phosphorylated eukaryotic elongation factor 2 | - |
? | |
ATP + [elongation factor 2] | the enzyme phosphorylates Ser595. Ser595 phosphorylation varies during the cell cycle and is required for efficient Thr56 phosphorylation (by elongation factor 2 kinase) in vivo | Homo sapiens | ADP + [elongation factor 2]phosphate | - |
? | |
ATP + [elongation factor 2] | the enzyme phosphorylates Ser595 | Homo sapiens | ADP + [elongation factor 2]phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDK2 | - |
Homo sapiens |
cyclin A-cyclin-dependent kinase 2 | - |
Homo sapiens |
cyclin-dependent kinase 2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
cyclin A | cyclin Acyclin-dependent enzyme. Phosphorylation of elongation factor 2 on Ser595 by the cyclin A-dependent enzyme directly stimulates phosphorylation of elongation factor 2 by elongation factor 2 kinase on Thr56 | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | S595 phosphorylation may globally couple the cell cycle machinery to regulatory pathways that impact eEF2K activity, eEF2 S595 phosphorylation is increased in mitosis. Cyclin A-CDK2 shuttles from the cytoplasm to the nucleus and could phosphorylate eEF2 in interphase cells | Homo sapiens |
physiological function | Ser595 phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase through T56 phosphorylation. S595 phosphorylation varies during the cell cycle and is required for efficient T56 phosphorylation in vivo, T56 phosphorylation inactivates eEF2 and is the only known normal eEF2 functional modification. S595 phosphorylation facilitates T56 phosphorylation by recruiting eEF2K to eEF2. | Homo sapiens |